Sjinkie Knegt (NED) and Charles Hamelin (CAN) failed to advance to the 500m quarterfinals.
Hamelin, a three-time Olympic gold medalist, has struggled in PyeongChang receiving penalties in all-three of his events. Hamelin will have one more chance to medal in the 5000m relay A Final.
"For sure, it is not the way I wanted to go but at the same time I cannot go hard on myself on that because there are still some races to go, for me and others from my teammates," Hamelin said following the race. "I need to keep up and make sure the vibe is still there with the team."
The 1000m is Knegt's final event of the 2018 Games. In PyeongChang, Knegt was able to add a 1500m silver medal to his 2010 1000m bronze.
"I wasn't really surprised by the decision. I just made a big mistake," Knegt said. "These kind of penalties are part of the sport. We know at the Games it's always different and this shows exactly why this is different."
Wu Dajing (CHN) was the bright spot of the heats, breaking the 500m Olympic-record with a time of 40.264 seconds.
All three Americans failed to advance to the quarterfinals.
Thomas Hong and John Henry Krueger finished third and fourth in their heats, respectively.
Aaron Tran finished third in his heat in his Olympuc debut, but was penalized after the race.