There will be no weightlifters representing Russia in the 2016 Rio Olympics.
The Court of Arbitration for Sport (CAS) rejected the country's appeal on Wednesday, upholding the ban put in place by the International Weightlifting Federation (IWF).
"The CAS panel in charge of this case has determined that the decision of the IWF was valid and proportionate to the circumstances," CAS secretary General Matthieu Reeb said.
"There have been criticisms about the CAS decision with respect to athletics a few days ago, but our activity, our job, is really to render decisions according to the law, we are not a political body, we do not express political opinions and of course we cannot be seen as a political institution.
"Maybe fans or the public in Russia may consider us as the bad guys but in reality it should not be so."
Eight Russian weightlifters were set to compete in the Rio Olympics before the ban.
"The integrity of the weightlifting sport has been seriously damaged on multiple times and levels by the Russians," the International Weightlifting Federation wrote in a July 29 statement. "Therefore, an appropriate sanction was applied in order to preserve the status of the sport."